Protein phosphorylation is a ubiquitous and key step in signaling pathways. For flow cytometry staining, the right protocol can help accurately capture these phosphorylated targets for clear-cut data.

Flow cytometry has revolutionized the research arena in numerous fields, including immunology, cancer biology, and cell biology. Flow cytometry possesses numerous advantages, including single-cell ...

Table 1 Antibody cocktails used for cell separation assessment by flow cytometry. Full size table In principle, flow cytometry is a system to measure and analyze the optical properties of single cells ...

In this case study, researchers at the Weizmann Institute of Science in Israel developed and optimized an imaging flow cytometry (IFC) protocol to provide a high-throughput, quantitative and highly ...

The interaction between cells in the central nervous system and immune cells are key in neuroinflammation, and play a role in the survival of neurons during neuropathology. Flow cytometry is a ...

High-throughput flow cytometry of adherent cells is difficult because the creation of single cell suspensions can damage cells and yield artificial results. We describe a protocol to increase the ...

Flow cytometry is a way to look closely at the features of cells or particles. A sample of blood or tissue goes into a machine called a cytometer. In less than a minute, a computer can analyze ...