Full-length RecE and RecT from Rac prophage mediate highly efficient linear–linear homologous recombination that can be used to clone large DNA regions directly from genomic DNA into expression ...

Young scientists often get frustrated with laborious experimental procedures, such as cloning, PCR, or sequencing. However, the Internet has made planning and performing these procedures faster and ...

Molecular cloning is a technique used to create DNA experimental tools that are expressed in either bacterial or mammalian cells. Molecular cloning is defined as the isolation and amplification of a ...

Molecular cloning refers to the replication and recombination of DNA molecules. The first cloning experiments were carried out in the 1970s, when restriction endonucleases were discovered. Restriction ...

Whether it’s heat, emotions, or stress, the body sweats it out through a varied mix of volatile chemicals and microbes. A large language model can design and troubleshoot CRISPR protocols from scratch ...

Forbes contributors publish independent expert analyses and insights. I cover global and domestic health care and conservation. This article is more than 3 years old. Twenty-five years ago, the ...